| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Clarksville Soccer Club Clarksville SC - B2005/06 Maroon |
0422-013B9U-0007 | Eric Ayisi |
| A2 | Emerald Force Soccer Club - 01 EFSC Dinamo Kiev 05/06 Boys |
0214-013B9U-0010 | Sevan Takvoryan |
| A3 | Emerald Force Soccer Club - 01 EFSC Leverkusen 2006 Boys |
0214-013B8U-0009 | Shawn Mehegan |
| A4 | FC Alliance Select Travel Teams FC Alliance 05/06 SL |
0204-01BB9U-0268 | Charles Perciful |
| A5 | Greater Cleveland Soccer Association Flames 2006 White |
0306-01CB8U-8950 | Gregory Celeste |
| A6 | Nashville United Soccer Academy - 01 NUSA B06 Gold |
0534-013B8U-0031 | Lamin Ceesay |
| A7 | Stones River Futbol Club - 01 Stones River FC 2005 Red |
0521-012B9U-0026 | Bouttavong Thompson |
| A8 | Tennessee Soccer Club TSC 2005/2006 Boys Premier |
0522-012B9U-2189 | Dalton Kaufman |
| A9 | One Knoxville One Knox 06/05B- Blue |
0293-01D3B9U-0055 | David Baisden |