| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 05/06 Boys |
0443-01SLB9U-0011 | Jose Perez |
| A2 | Emerald Force Soccer Club - 01 EFSC Shaktar 05/06 Boys |
0214-012B9U-0004 | Sevan Takvoryan |
| A3 | Greeneville Soccer Association Greeneville Soccer Association - B19U |
0102-013B9U-7831 | Nicolas Ranieri |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2005/2006 Boys Showcase |
0522-012B9U-2252 | Nilson Meza |
| A5 | One Knoxville One Knox 06/05B- Orange |
0293-01D2B9U-0027 | Antioco Castaneda Varela |
| A6 | Tennessee Premier Soccer Academy PSA Lanus TSL |
0317-022B9U-0061 | Paul Furey |