| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy CSM Forge U18 Boys |
0316-022B8U-7959 | Matthew Jelley |
| A2 | Greater Cleveland Soccer Association Flames 2006 Maroon |
0306-01CB8U-8949 | Leo Arntz |
| A3 | Knoxville United Soccer Association - 01 KUSA 06B Stars |
0210-012B8U-0001 | Francis Colbert |
| A4 | Camp Forest Football Club Camp Forest FC 2006 Boys |
0523-022B8U-0002 | Andrew Sain |
| A5 | Action Elite Soccer Club (formerly known as Action Indoor Sports Soccer Assn.) Action Elite-06/07 |
0501-012B8U-1313 | Curtis Dewolfe |
| A6 | Wilson United Soccer Club Wilson United SC 2006 B Black |
0406-012B8U-0005 | Simon Aldave |