| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Select Soccer Academy Tennessee Sel - B18U |
0213-01CB8U-0395 | Scott Muir |
| A2 | Music City Soccer Club Music City FC 06B Premier |
0517-011B8U-3499 | Jose Hernandez |
| A3 | Tennessee United Soccer Club TUSC 2006 Boys NPL |
0424-011B8U-0011 | Nathan Sego |
| A4 | Chattanooga FC Academy Chattanooga FC - 2006B Navy |
0316-021B8U-7936 | Andrew Wishart |
| A5 | Stones River Futbol Club - 01 Stones River FC 2006 Black |
0521-011B8U-0023 | Bouttavong Thompson |
| A6 | Clarksville Soccer Club Clarksville SC - B2006 Maroon |
0422-011B8U-0006 | Scott Dollar |
| A7 | Tennessee Soccer Club TSC Nashville 2006 Boys Showcase |
0522-012B8U-0066 | Bryan Eoff |
| A8 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B8U-0012 | Ethan Keogh |
| A9 | Tri-Cities United Soccer Club Tri-Cities United 06 Boys Premier |
0106-011B8U-0009 | Bracken Burns |