| Group | Club/Team Name | Team ID | Head Coach |
| A1 | ALBION SC Nashville (Formerly Southern Premier Soccer) ALBION SC Nashville - B07 Academy |
0540-02D2B7U-0037 | Stephen Fawehinmi |
| A2 | Aris FC Aris FC - 2007 Boys Black |
0530-022B7U-0153 | John Petrou |
| A3 | Kings Hammer KHM 07/08B Premier I |
0515-012B7U-0211 | Blake Moore |
| A4 | Nashville United Soccer Academy - 01 NUSA B07 Navy |
0534-012B7U-0024 | Fitzroy Charles |
| A5 | Stones River Futbol Club - 01 Stones River FC 2007 Black |
0521-012B7U-0021 | Thomas Williams |
| A6 | Tennessee Soccer Club TSC Murfreesboro 2007 Boys Premier |
0522-012B7U-2191 | Eli Wellman |
| A7 | Wilson United Soccer Club Wilson United SC 2007 B Black |
0406-012B7U-0004 | Robert Jepson |
| A8 | Tennessee Soccer Club TSC Williamson 2007 Boys Premier |
0522-013B7U-2196 | Leonardo Santamaria |