| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 07 Boys |
0443-01SLB7U-0010 | Stone Boston |
| A2 | Blount United Soccer Club Blount United 2007 Hammers |
0201-012B7U-0228 | Juan Juma |
| A3 | FC Alliance Select Travel Teams FC Alliance 2007B RL |
0204-012B7U-0264 | Edgard Semaan |
| A4 | One Knoxville One Knox 07B- Blue |
0293-01D2B7U-0029 | Gustavo Alvarez-Suchini |
| A5 | One Knoxville One Knox 07B- Orange |
0293-01D2B7U-0026 | Tanner Hopwood |
| A6 | Tennessee Premier Soccer Academy PSA Pedro Jrs TSL |
0317-022B7U-0060 | Paul Furey |
| A7 | Tri-Cities United Soccer Club Tri-Cities United 07 Boys Premier |
0106-011B7U-0008 | Dustin Deal |
| A8 | Redoubt Soccer Association - 01 2007 Redoubt Generals |
0312-013B7U-0008 | Chaz Jackson |