| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Columbia Soccer Association - 01 2008 Columbia Arsenal |
0504-013B6U-0005 | John Spencer |
| A2 | Lobos Rush (Collierville Soccer Association) 2008 Lobos Rush Elite |
0603-013B6U-0040 | Walter Aeschliman |
| A3 | Germantown Legends Germantown Legends 08 Red |
0606-013B6U-0024 | Thomas Barlow |
| A4 | Tennessee Soccer Club TSC Williamson 2008 Boys Elite |
0522-013B6U-2200 | Matheus Mello |
| A5 | Midsouth Futbol Midsouth 2008 Boys Showcase |
0654-013B6U-0104 | Chad Reynolds |
| A6 | Pateadores Soccer Club - Nashville Pats U16 White |
0500-01D3B6U-0011 | Eric Montufar |
| A7 | Stones River Futbol Club - 01 Stones River FC 2008 Black |
0521-012B6U-0017 | William Vice |
| A8 | Tennessee United Soccer Club TUSC 2008 Boys Blue |
0424-013B6U-0025 | Matthew Ohlwein |