| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 2008B Red |
0324-022B6U-0006 | Jim Cofer |
| A2 | Chattanooga FC Academy CSM Forge U16 Boys |
0316-022B6U-7958 | Cristian Toledano-Gonzalez |
| A3 | Emerald Force Soccer Club - 01 EFSC Chelsea 2008 Boys |
0214-012B6U-0003 | Kirk McClelland |
| A4 | FC Alliance Select Travel Teams FC Alliance 2008B RL |
0204-012B6U-0265 | Edgard Semaan |
| A6 | Johnson City Futbol Club - 01 JCFC 2008B Black |
0216-012B6U-0002 | Neal Lewis |
| A7 | One Knoxville One Knox 08B- Orange |
0293-01D2B6U-0010 | Josiah MacMillan |
| A8 | Tri-Cities United Soccer Club Tri-Cities United 08/09 Boys Premier |
0106-012B6U-0007 | Jordan Campbell |
| A9 | Redoubt Soccer Association - 01 2008 Redoubt Generals |
0312-013B6U-0002 | Doug Allen |