| Group | Club/Team Name | Team ID | Head Coach |
| A1 | TN Tempo FC Academy TN Tempo FC Academy - 2008B |
0592-011B6U-0010 | Sunebari Ndam |
| A2 | Tennessee United Soccer Club TUSC 2008 Boys NPL |
0424-011B6U-0009 | Nduka Kemjika |
| A3 | Pateadores Soccer Club - Nashville Pats 2008 Blue |
0500-01D1B6U-0005 | n/a |
| A4 | Nashville United Soccer Academy - 01 NUSA B08 Maroon |
0534-011B6U-0010 | Graeme Boyle |
| A5 | Tennessee Soccer Club TSC Williamson 2008 Boys Showcase |
0522-012B6U-0063 | Stephen Watters |
| A6 | Pateadores Soccer Club - Nashville Pats 2008 Yellow |
0500-01D1B6U-0004 | Daniel Suarez |
| A7 | Lobos Rush (Collierville Soccer Association) 2008 Lobos Rush ECNL |
0603-011B6U-0006 | Brandon Carter |
| A8 | Tennessee Soccer Club TSC Murfreesboro 2008 Boys Showcase |
0522-011B6U-2197 | Christian Dale |
| A9 | One Knoxville One Knox 08B- NPL |
0293-01D1B6U-0024 | Saahil Kapoor |