| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 09/10B Conejos |
0443-01SLB5U-0008 | Jonathan Anderson |
| A2 | AYSO Alliance Knoxville - 01 AYSOR124 ALLIANCE 09B |
0292-013B5U-0027 | Kevin Davis |
| A3 | Blount United Soccer Club Blount United 2009 Hammers |
0201-013B5U-0231 | Ethan Brass |
| A5 | Chattanooga FC Academy Chattanooga FC - 2009B White |
0316-023B5U-7932 | Thomas Bentham |
| A6 | Emerald Force Soccer Club - 01 EFSC Bayern 2009 Boys |
0214-013B5U-0006 | Lloyd Brennan |
| A7 | Greater Cleveland Soccer Association Flames 2009 Maroon |
0306-01CB5U-8952 | Gabriel Harden |
| A8 | One Knoxville One Knox 09B- Blue |
0293-01D3B5U-0053 | Thomas Bartyzel |
| A9 | Rhea County Football Club Play (RC3) Rc3 FC Rampage |
0322-01D3B5U-0041 | Jeremy Toliver |