| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2009 Lobos Rush E64 |
0603-012B5U-0052 | Christopher Wright |
| A2 | Kononia Soccer Club - 01 Blues City FC 2009 |
0611-012B5U-1986 | Merlyn Baltodano |
| A3 | Clarksville Soccer Club Clarksville SC - B2009 Maroon |
0422-012B5U-0003 | Alex Villa |
| A4 | Germantown Legends Germantown Legends 09 Red |
0606-012B5U-0022 | Carey Talley |
| A5 | Jackson Soccer Club JSC 2009 Boys Gold |
0609-012B5U-0012 | Malang Jarju |
| A6 | Midsouth Futbol Midsouth Bartlett Premier 2009 Boys |
0654-012B5U-0111 | John Stoever |
| A7 | Soccer Ole OLE FC 09B Premier |
0632-022B5U-0014 | Evan Hacker |
| A8 | Tennessee Soccer Club TSC Nashville 2009 Boys Showcase |
0522-012B5U-2249 | Lover High |
| A9 | Tennessee Soccer Club TSC Williamson 2009 Boys Showcase |
0522-012B5U-0061 | Ruben Arcega |