| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Aris FC Aris FC - 2009 Boys |
0530-022B5U-0151 | John Petrou |
| A2 | Kings Hammer KHM 09B Premier I |
0515-012B5U-0208 | Paul Parkerson |
| A3 | Nashville United Soccer Academy - 01 NUSA B09 Navy |
0534-012B5U-0022 | n/a |
| A4 | Pateadores Soccer Club - Nashville Pats 2009 Yellow |
0500-01D2B5U-0007 | Sergio Otalora |
| A5 | Stones River Futbol Club - 01 Stones River FC 2009 Red |
0521-012B5U-0016 | Raul Lopez |
| A6 | Tennessee Soccer Club TSC Murfreesboro 2009 Boys Showcase |
0522-012B5U-2204 | Enrique Suarez |
| A7 | Tennessee Soccer Club TSC Williamson 2009 Boys Premier |
0522-012B5U-2210 | Peter Russotto |
| A8 | Tennessee United Soccer Club TUSC 2009 Boys Red |
0424-012B5U-0018 | Jamie Smith |
| A9 | TN Tempo FC Academy TN Tempo FC Academy - 2009B |
0592-012B5U-0013 | Sunebari Ndam |