| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2009B Navy |
0316-022B5U-7931 | Benjamin Wilkinson |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 2009B Red |
0324-022B5U-0005 | Nicholas Davis |
| A3 | Emerald Force Soccer Club - 01 EFSC Man City 2009 Boys |
0214-012B5U-0002 | Robert Gibson |
| A4 | FC Alliance Select Travel Teams FC Alliance 2009B RL |
0204-012B5U-0266 | Erik Casterline |
| A5 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B5U-0129 | Chris Burks |
| A6 | One Knoxville One Knox 09B- Orange |
0293-01D2B5U-0023 | Brenna Lacaillade |
| A7 | Soccer Club of Oak Ridge - 01 SCOR 09 Boys |
0208-012B5U-0004 | Seua Moua |
| A8 | Redoubt Soccer Association - 01 2009 Redoubt Generals |
0312-013B5U-0009 | Richard Tate |