| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Pateadores Soccer Club - Nashville Pats 2009 Blue |
0500-01D1B5U-0003 | Maria Jose Harispuru |
| A2 | Stones River Futbol Club - 01 Stones River FC 2009 Gray |
0521-011B5U-0014 | Bernardo Ortega |
| A3 | Tennessee United Soccer Club TUSC 2009 Boys NPL |
0424-011B5U-0008 | Nathan Sego |
| A4 | All-In FC TN All-In FC TN 09B Chippewas |
0443-01SLB5U-0007 | Davis Draper |
| A5 | One Knoxville One Knox 09B- NPL |
0293-01D1B5U-0009 | Jordan Bell |
| A6 | Germantown Legends Germantown Legends 09 White |
0606-011B5U-0007 | Antonio Carbognani |
| A7 | Germantown Legends Germantown Legends 09 Black |
0606-011B5U-0008 | Carey Talley |
| A8 | Nashville United Soccer Academy - 01 NUSA B09 Maroon |
0534-011B5U-0009 | Jared MacLane |