| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2010B White |
0316-023B4U-7929 | Casey Johnston |
| A2 | Emerald Force Soccer Club - 01 EFSC AC Milan 2010 Boys |
0214-013B4U-0005 | Jonathan Johnson |
| A3 | One Knoxville One Knox 10B- Blue |
0293-01D3B4U-0052 | Thomas Bartyzel |
| A4 | Tri-Cities United Soccer Club Tri-Cities United 2010 Boys Premier |
0106-011B4U-0006 | Ovidiu Chirica |
| A5 | USA North USA North - B 2010 |
0218-01D3B4U-0008 | Darrick Lubell |
| A6 | Wilson United Soccer Club Wilson United SC 2010 B Red |
0406-013B4U-0011 | Nicole Eichberger |
| A7 | TN Tempo FC Academy TN Tempo FC Academy - 2010B |
0592-012B4U-0012 | Nathaniel Bedai |
| A8 | Stones River Futbol Club - 01 Stones River FC 2010 White |
0521-013B4U-0013 | Jomo Cromwell |
| A9 | Kings Hammer KHM 10B Premier II |
0515-013B4U-0207 | Ali Arman |
| A10 | Tennessee United Soccer Club TUSC 2010 Boys Blue |
0424-013B4U-0024 | Brian McKee |