| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Columbia Soccer Association - 01 2010 Columbia Arsenal |
0504-012B4U-0004 | Rey Martinez |
| A2 | ALBION SC Nashville (Formerly Southern Premier Soccer) ALBION SC Nashville - B10 Academy |
0540-02D2B4U-0035 | Brent Goulet |
| A3 | Armada Futbol Club Armada FC 2010 Boys Red |
0436-012B4U-0009 | Mirgen Bushati |
| A4 | Clarksville Soccer Club Clarksville SC - B2010 Maroon |
0422-012B4U-0011 | Alejandro Herrera |
| A5 | Nashville United Soccer Academy - 01 NUSA B10 Navy |
0534-012B4U-0021 | Julian Lucumi Villegas |
| A6 | Pateadores Soccer Club - Nashville Pats 2010 Yellow |
0500-01D2B4U-0006 | Nocturne Dsilva |
| A7 | Tennessee Soccer Club TSC Williamson 2010 Boys Showcase |
0522-012B4U-2223 | Michael Alley |
| A8 | Tennessee United Soccer Club TUSC 2010 Boys Red |
0424-012B4U-0017 | John Lehn |