| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2012B Navy |
0316-02SJB2U-7921 | Dalton Healy |
| A2 | FC Alliance ChallengeTeams FC Alliance 2013B Pre-ECNL |
0204-02SB1U-0227 | Caleb Lucas |
| A3 | Kings Hammer KHM 12B Premier I |
0515-01DB2U-0203 | Brian Grogan |
| A4 | Nashville United Soccer Academy - 01 NUSA B12 Maroon |
0534-01AB2U-0047 | Calvin Da Cunha |
| A5 | Tennessee Soccer Club TSC Williamson 2013 Boys Academy Premier Blue |
0522-01AB1U-2262 | James Drain |